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KMID : 0357220110230050035
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Journal of Korean Society Physical Therapy
2011 Volume.23 No. 5 p.35 ~ p.41
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Localization of Amygdaloid Nucleus Innervating the Stomach Using Genetically Engineered PRV-BaBlu in Rat Brain
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Song Ju-Min
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Abstract
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Purpose: This study was carried out to investigate the spatiotemporal localization of the amygdaloid nucleus innervating the rat stomach using PRV?BaBlu, which has been known to be an excellent type of neurotracer with the ability to transpass the neuronalsynaptic cleft.
Methods: Ninety Sprague?Dawley rats (250~300 g) that were injected with PRV?BaBlu into the stomach were randomly divided into 3, 4 and 5 day groups (each group n=30). 2 μl of PRV?BaBlu, a genetically modified strain of PRV?Bartha with the lac?Z gene,was injected into the rat stomach and immunostained with a mouse anti?β?galactosidase at 3, 4 and 5 days after the virus injection.
Results: The PRV?BaBlu infected the neurons in the amygdaloid nucleus, and the degree of viral infection in experimental animals showed a tendency to increase significantly with time (p<0.05). The neurons between the left and right amygdaloid nucleus significantly differ (p<0.05).
Conclusion: This showed that PRV?BaBlu was an excellent neurotracer for localizing the amygdaloid nucleus, and the amygdaloid nucleus has a sensory input and motor output on stomach movement, influencing emotional behavior.
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KEYWORD
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PRV-BaBlu, Amygdaloid nucleus, Stomach, Immunostain
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